方可 講師 碩士生導(dǎo)師
E-mail: [email protected]
辦公地點(diǎn)：李文正樓北235
個(gè)人簡介：
2012年和2018年于中山大學(xué)生命科學(xué)學(xué)院獲得學(xué)士和博士學(xué)位，2018-2021年于中山大學(xué)生命科學(xué)學(xué)院進(jìn)行博士后研究。2021年加入東南大學(xué)生命科學(xué)與技術(shù)學(xué)院，任講師，碩士生導(dǎo)師。共發(fā)表SCI論文20余篇，其中以第一或通訊作者發(fā)表在高水平期刊如Cell death and differentiation、 Genome Biology、Journal of Hematology & Oncology 、Clinical and Translational Medicine上。主持國家自然科學(xué)基金面上項(xiàng)目、國家自然科學(xué)基金青年基金、中國博士后面上項(xiàng)目(一等資助)，入選江蘇省“雙創(chuàng)博士”和東南大學(xué)“至善學(xué)者”人才計(jì)劃。
研究方向：表觀遺傳及轉(zhuǎn)錄調(diào)控在細(xì)胞命運(yùn)決定中的功能機(jī)制
1. 表觀遺傳及轉(zhuǎn)錄調(diào)控介導(dǎo)細(xì)胞命運(yùn)決定的功能機(jī)制
基因表達(dá)的改變是細(xì)胞命運(yùn)決定及個(gè)體命運(yùn)決定最關(guān)鍵的途徑。特定基因座位的表觀遺傳改變，進(jìn)而介導(dǎo)的轉(zhuǎn)錄差異是細(xì)胞命運(yùn)決定的核心。我們致力于探究基因座位表觀遺傳的改變（如結(jié)構(gòu)性改變（G4/R-loop），修飾性改變等(組蛋白修飾等)）與基因轉(zhuǎn)錄的關(guān)系，并在發(fā)育（如心臟發(fā)育）及疾?。ㄈ绨┌Y）模型中探究其對細(xì)胞命運(yùn)決定的調(diào)控作用。
2. 轉(zhuǎn)錄調(diào)控在疾病中的功能機(jī)制及靶向治療
癌基因，抑癌基因等的轉(zhuǎn)錄調(diào)控異常往往直接介導(dǎo)的癌癥產(chǎn)生。我們聚焦于癌癥相關(guān)基因的轉(zhuǎn)錄調(diào)控，尋找癌癥發(fā)生發(fā)展的重要調(diào)控因子，揭示其細(xì)胞功能，如不同細(xì)胞死亡調(diào)控的切換等。并以此為基礎(chǔ)開發(fā)潛在的靶向治療策略。
發(fā)表論文：
1.Zhao X*, Fang K*, Liu X*, Yao R, Wang M, Li F, Hao S, He J, Wang Y, Fan M, Huang W, Li Y, Gao C, Lin C, Luo Z. QSER1 Preserves the Suppressive Status of the Pro-Apoptotic Genes to Prevent Apoptosis[J]. Cell Death and Differentiation, 2023, 30(3):779-793.
2.Huang W, Zeng ZC, Wang WT, Sun YM, Chen YQ, Luo XQ, Fang K#. A CRISPR/CAS9-based strategy targets the personalized chimeric neosequence in fusion-driven cancer genome for precision medicine. Clinical and Translational Medicine 2021, 11(3): e355.
3.Fang K*, Huang W*, Sun YM, Chen TQ, Zeng ZC, Yang QQ, Pan Q, Han C, Sun LY, Luo XQ, Wang WT, Chen YQ. Cis-acting lnc-eRNA SEELA directly binds histone H4 to promote histone recognition and leukemia progression. Genome Biology 2020, 21(1): 269.
4.Huang W*, Fang K*, Chen TQ, Zeng ZC, Sun YM, Han C, Sun LY, Chen ZH, Yang QQ, Pan Q, Luo XQ, Wang WT, Chen YQ. circRNA circAF4 functions as an oncogene to regulate MLL-AF4 fusion protein expression and inhibit MLL leukemia progression. J HEMATOL ONCOL 2019, 12(1): 103.
5. Meng S, Liu X, Zhu S, Xie P, Fang H, Pan Q, Fang K, Li F, Zhang J, Che Z, Zhang Q, Mao G, Wang Y, Hu P, Chen K, Sun F, Xie W, Luo Z, Lin C. Young LINE-1 Transposon 5' UTRs Marked by Elongation Factor ELL3 Function as Enhancers to Regulate Naive Pluripotency in Embryonic Stem Cells[J]. Nature Cell Biology, 2023, 25(9):1319-1331
6.Che Z, Liu X, Dai Q, Fang K, Guo C, Yue J, Fang H, Xie P, Luo Z, Lin C. Distinct Roles of the Two SEC Scaffold Proteins, AFF1 and AFF4, in Regulating RNA Pol II Transcription Elongation[J]. Journal of Molecular Cell Biology, 2023.
7.Zeng Z C, Pan Q, Sun Y M, Huang H J, Chen X T, Chen T Q, He B, Ye H, Zhu S X, Pu K J, Fang K, Huang W, Chen Y Q, Wang W T. METTL3 Protects METTL14 From STUB1-mediated Degradation to Maintain M(6) a Homeostasis[J]. Embo Reports, 2023, 24(3):e55762.
8. Han C, Sun LY, Luo XQ, Pan Q, Sun YM, Zeng ZC, Chen TQ, Huang W, Fang K, Wang WT, Chen YQ. Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. CELL REP 2022, 38(13): 110421.
9.Huang W, Chen TQ, Fang K, Zeng ZC, Ye H, Chen YQ. N6-methyladenosine methyltransferases: functions, regulation, and clinical potential. J HEMATOL ONCOL 2021, 14(1): 117. (中科院一區(qū)；IF=17.4，五年IF=12.6)
10. Sun L, Wang W, Han C, Huang W, Sun Y, Fang K, Zeng Z, Yang Q, Pan Q, Chen T, Luo X, Chen Y. The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. MOL CELL 2021, 81(21): 4493-4508.
11. Chen ZH, Chen TQ, Zeng ZC, Wang D, Han C, Sun YM, Huang W, Sun LY, Fang K, Chen YQ, Luo XQ, Wang WT. Nuclear export of chimeric mRNAs depends on an lncRNA-triggered autoregulatory loop in blood malignancies. CELL DEATH DIS 2020, 11(7): 566.
12. Wang WT, Chen TQ, Zeng ZC, Pan Q, Huang W, Han C, Fang K, Sun LY, Yang QQ, Wang D, Luo XQ, Sun YM, Chen YQ. The lncRNA LAMP5-AS1 drives leukemia cell stemness by directly modulating DOT1L methyltransferase activity in MLL leukemia. J HEMATOL ONCOL 2020, 13(1): 78.
13. Sun YM, Wang WT, Zeng ZC, Chen TQ, Han C, Pan Q, Huang W, Fang K, Sun LY, Zhou YF, Luo XQ, Luo C, Du X, Chen YQ. circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression. BLOOD 2019, 134(18): 1533-1546.
14.Wang WT, Han C, Sun YM, Chen ZH, Fang K, Huang W, Sun LY, Zeng ZC, Luo XQ, Chen YQ. Activation of the Lysosome-Associated Membrane Protein LAMP5 by DOT1L Serves as a Bodyguard for MLL Fusion Oncoproteins to Evade Degradation in Leukemia. CLIN CANCER RES 2019, 25(9): 2795-2808.
15.Sun LY, Li XJ, Sun YM, Huang W, Fang K, Han C, Chen ZH, Luo XQ, Chen YQ, Wang WT. LncRNA ANRIL regulates AML development through modulating the glucose metabolism pathway of AdipoR1/AMPK/SIRT1. MOL CANCER 2018, 17(1): 127.
16.Huang W, Wang WT, Fang K, Chen ZH, Sun YM, Han C, Sun LY, Luo XQ, Chen YQ. MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47. MOL CANCER 2018, 17(1):
17.Chen ZH, Wang WT, Huang W, Fang K, Sun YM, Liu SR, Luo XQ, Chen YQ. The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway. CELL DEATH DIFFER 2017, 24(2): 212-224.
18.Fang K*, Han BW*, Chen ZH, Lin KY, Zeng CW, Li XJ, Li JH, Luo XQ, Chen YQ. A distinct set of long non-coding RNAs in childhood MLL-rearranged acute lymphoblastic leukemia: biology and epigenetic target. HUM MOL GENET 2014, 23(12): 3278-3288.
19.Duan FT, Qian F, Fang K, Lin KY, Wang WT, Chen YQ. miR-133b, a muscle-specific microRNA, is a novel prognostic marker that participates in the progression of human colorectal cancer via regulation of CXCR4 expression. MOL CANCER 2013, 12: 164.
20.Fang K, Qian F, Chen YQ. MicroRNAs as regulators in normal hematopoietic and leukemia stem cells: current concepts and clinical implications. Current Molecular Medicine 2012 Jun,12(5):536-46.
